What is Chromatography and it's types? Do you know the applications of Chromatography?
CHROMATOGRAPHY is the technique for the separation, purification, and testing of compounds. The term "chromatography" is derived from Greek, chroma meaning, "color", and graphein meaning " to write". In this process, we use the mixture to be separated on a stationary phase (solid or liquid) and a pure solvent such as water or any gas is allowed to move slowly over the stationary phase, carrying the components separately as per their solubility in the pure solvent. Principles of Chromatography chromatography is a separation method where the analyte is combined within a liquid or gaseous mobile phase, which is pumped through a stationary phase. Usually, one phase is a hydrophilic and the other lipophilic. The component of the analyte interacts differently with these two phases. Depending on their polarity they spend more or less time interacting with the stationary and are thus retarded to a greater or lesser extent. This leads to a separation of the differ